Oct 18, 2023
Optical densitometry of neuronal fibers
- 1Vall d'Hebron Research Institute
Protocol Citation: Miquel Vila 2023. Optical densitometry of neuronal fibers. protocols.io https://dx.doi.org/10.17504/protocols.io.8epv5x3nng1b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 18, 2023
Last Modified: November 20, 2023
Protocol Integer ID: 89505
Abstract
Optical densitometry of striatal neuronal fibers in the rodent brain
Scan the immunostained serial coronal sections covering different caudorostral levels of the brain regions (for striatum, 4 sections/animal) with an Epson Perfection v750 Pro scanner.
With the free hand selections tool, draw the region of interest (e.g. striatum, nucleus accumbens and olfactory tubercle) and measure the optical density (pixel brightness). Then draw a region of interest in a blank area (e.g. cortex) and also measure the optical density.
Calculate the optical density or absorbance using the formula: -log10 (Striatum Intensity/Cortex Intensity).