Jun 09, 2023
Open Field test to assess spontaneous locomotion behavior in parkinsonian mice
- natalia.lopezgonzalezdelrey1,
- Zachary Gaertner1
- 1Northwestern University, Feinberg School of Medicine, Neurology Department
Protocol Citation: natalia.lopezgonzalezdelrey, Zachary Gaertner 2023. Open Field test to assess spontaneous locomotion behavior in parkinsonian mice. protocols.io https://dx.doi.org/10.17504/protocols.io.n92ldmbrol5b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 09, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 83159
Keywords: ASAPCRN, Behavior Test, Open Field, parkinsonian behavior
Funders Acknowledgement:
Aligning Science Across Parkinson's [ASAP-020600] through the Michael J. Fox Foundation for Parkinson's Research (MJFF)
Grant ID: ASAP-020600
Abstract
The Open Field task is a simple sensorimotor test used to determine general activity levels, gross locomotor activity, and exploration habits in rodent models of CNS disorders.
Materials
- Square, white Plexiglas box
- Overhead camera.
Open Field Test
Open Field Test
1d
1d
Total duration: 1 day
1d
Protocol
Protocol
1h 5m
1h 5m
Place the animal cage in the testing room to allow the animals to acclimate. 01:00:00
1h
Place the animal in the testing boxes. The animals are transferred to the center of a 40cm by 40cm box in the quiet dark room.
5m
Video record and record freely moving behavior in the testing cage.00:05:00
5m
Return the mouse to its cage.
1m
Analysis
Analysis
The footage is then analyzed by an automated tracking system for the following parameters: distance moved, velocity, and time spent in pre-defined zones.