Feb 14, 2024
Open field test in rats
- Eduard Bentea1,
- María Sanchiz Calvo1,
- Veerle Baekelandt1
- 1Katholieke Universiteit Leuven
Protocol Citation: Eduard Bentea, María Sanchiz Calvo, Veerle Baekelandt 2024. Open field test in rats. protocols.io https://dx.doi.org/10.17504/protocols.io.kxygx3dozg8j/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 06, 2024
Last Modified: May 31, 2024
Protocol Integer ID: 94756
Keywords: ASAPCRN, motor function, behavioral test, rats
Funders Acknowledgement:
ASAP (Aligning Science Across Parkinson's)
Abstract
Protocol for performing the open field test in rats. The open field test measures spontaneous locomotion during 5-min free exploration of an open field arena (1m x 1m), and can be used to measure hypolocomotion in animal models of Parkinson's disease. Rats with decreased nigrostriatal dopaminergic neurotransmission show decreased mobility during the open field test.
Test
Test
1h 5m
Bring cages to the behavioral room for at least 01:00:00 prior to the test for habituation
1h
Place rat individually in a clean open field arena (1m x 1m)
Video tape from above for 00:05:00 and record parameters of spontaneous activity (distance traveled, movement time, immobility) using an activity tracking software.
5m
Clean arena with mild soap solution