Dec 01, 2023
Open field test
- 1Massachusetts General Hospital, ASAP
Protocol Citation: Pranay Srivastava 2023. Open field test. protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvj3bxnlk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 30, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 91663
Keywords: ASAPCRN
Abstract
Open field test can be used to evaluate locomotor activity in rodents.
Prepare the open field arena.
Allow the animal to acclimate to the testing environment for one hour.
The mice were placed in the plexiglass chamber (11 × 11 in with clear 8-in high walls) and were allowed to explore for a period of 10 min.
The total distance travelled was measured with software Ethovision XT 9.0, Noldus Information Technology, The Netherlands.