Dec 14, 2023
Open Field Protocol
- 1University of Minnesota
Protocol Citation: Michael Lee 2023. Open Field Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.36wgq7qjovk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 07, 2022
Last Modified: May 31, 2024
Protocol Integer ID: 64066
Keywords: Open Field Protocol, locomotor activity, ASAPCRN
Funders Acknowledgement:
ASAP
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Abstract
This protocol details about Open Field procedure.
Attachments
452-957.docx
16KB
Guidelines
Detailed records should be kept on every experiment, noting date, timing, subject test order, procedural details followed and any relevant observations pertaining to the assay (e.g. specific behavior or procedural errors).
Set up
Set up
20 x 20 x 10 inch chamber (LxWxH).
Opaque chambers or dividers between chambers.
Light intensity = 250 lux.
Have chambers on enclosed rack to diminish outside light.
Test
Test
30m
AnyMaze
Set two zones in the chamber. Outer ~3.5 inch perimeter of box is one zone and the center of the maze is the other zone.
Record locomotor activity and track how much time the animal spends in each zone for 00:30:00 .
30m
Wipe down chambers with ethanol between animals.
Run males before females if running both genders on same day.
Can be used as a simple locomotion test by tracking the distance traveled and speed.
Can also be used as an anxiety test. Less anxious animals will spend more time in the center of the maze.