If you have access to a Quantus/Qubit fluorometer, now is a good time to quantify the resulting amount of DNA in your purified sample.
You are looking to be around 1ug DNA per 50uL water as an end goal. Each plate of end-product contains approximately the following amount of DNA with this protocol, assuming a 500uL subsample was taken near the beginning of this protocol.
Assuming 100uL of water added:
Promega Wizard Extraction - results in ~63 ng/uL (6,300ng)
X-Amp extraction of dried tissues - results in ~72 ng/uL (7,200 ng)
X-Amp extraction of fresh tissues - results in ~73 ng/uL (7,300 ng)
These numbers are just for a reference. The numbers could be different depending on the type of tissue being used, extraction method being used, and the PCR program employed.
Further trials showed a typical final concentration of 86 - 108 ng/uL with 5-10 plates of PCR product combined of X-amp extractions from dried tissue.
So for the final end product:
There are 1000ng in a ug. 1000/90 = 11.1uL to get to 1ug in the final sample.
In a new 1.5uL eppi tube combine 11.1uL of the resulting diluted DNA solution combined with 38.9uL (=50 minus 11.1) of water for the next step. (1ug DNA per 50uL water).
This is what I would utilize if you do not have the ability to accurately quantify DNA.
If you can quantify, just use your final concentration in the calculation.
1000/108 = 9.3uL template
Final in a new 1.5uL eppi tube: 9.3uL template + 40.7uL of water.