Dec 10, 2024
OGI Bio Liquid Control Tubing Cleaning
- 1OGI Bio
- OGI Bio
Protocol Citation: Jack Hocking 2024. OGI Bio Liquid Control Tubing Cleaning . protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gpz2jdlzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 08, 2024
Last Modified: December 10, 2024
Protocol Integer ID: 104961
Disclaimer
The cleaning protocols provided by OGI Bio are intended as general guidelines for the maintenance of our micro bioreactor equipment. These protocols are designed to ensure optimal performance and longevity of the equipment under standard operating conditions.
OGI Bio assumes no liability for any damage, contamination, or malfunctions resulting from deviations from these protocols, improper use, or the use of incompatible cleaning agents or methods. It is the responsibility of the user to verify that all cleaning agents and procedures are compatible with local regulations, organizational standards, and the materials of the equipment in use.
Abstract
The protocol for the care and cleaning of your OGI BioReactor Liquid Control Tubing.
Guidelines
The tubing should be cleaned immediately after an experiment finishes to prevent fouling due to dry culture.
The tubing should be replaced after 5 autoclave cycles.
Materials
70% Ethanol Contributed by users
Ultrapure WaterContributed by users
Equipment
Liquid Control Tubing
NAME
Accessory
TYPE
ŌGI Bio
BRAND
A200020
SKU
LINK
ID/OD: 1 mm / 3 mm
SPECIFICATIONS
Before start
Ensure you have familiarised yourself with the guidelines and warnings for this protocol.
Cleaning
Cleaning
Immediately after an experiment, run the Pump Cleaning protocol on your OGI BioReactor by selecting Pump Cleaning > Start Cleaning in the BioReactor menu.
Put the input ends of the tubing (on the right-hand side of each pump) in a bottle with 70% ethanol, and the output ends in an empty bottle.
Follow the instructions on the LCD screen to turn the pumps on and pump ethanol through the tubing for at least 30 seconds.
Stop the pumps as instructed on the LCD screen and swap the bottle with ethanol for a bottle with ultrapure water.
Follow the instructions on the LCD screen to turn the pumps on and pump water through the tubing for at least 60 seconds.
Without turning the pumps off, remove the input ends of the tubing from the water and let the pumps run until there is no more fluid coming out of the output ends.
Follow the instructions on the LCD screen to stop the pumps.
Sterilisation
Sterilisation
Prior to autoclave-sterilization, cover the ends of the tubing in tinfoil and secure with autoclave tape.
Autoclave at 121 °C, do not remove the tinfoil covering the ends until the tubing needs to be used again. This ensures that the ends of the tubing remain sterile until use.
The tubing should be replaced after 5 autoclave cycles.