Protocol Citation: sdwalto, Jeffrey Kordower, Bryan Killinger 2024. Odor Threshold. protocols.io https://dx.doi.org/10.17504/protocols.io.kqdg3xm37g25/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 28, 2024
Last Modified: May 31, 2024
Protocol Integer ID: 95892
Keywords: ASAPCRN, mice behavior, odor threshold
Funders Acknowledgement:
Jeffrey Kordower
Grant ID: NIH R21 NS109871
Abstract
Odor threshold optimized for mice. This test was made to test short term olfactory memory. Mice with olfactory impairment or deficits should score lower.
Guidelines
ODORANT: Propionic acid at increasing concentrations of 1:106, 1:104, and 1:103 diluted in mineral oil.
Notes:
- Paper swabs soaked in the different odorant dilutions were used inside the cartridges.
- Investigation time defined as the duration of active sniffing with the nose placed less than 1 cm away from the cartridge.
Prepare and empty rodent cage with no bedding.
Prepare the odorant by diluting in mineral oil. (See guidelines)
Acclimate mouse to setup (empty cage with odor cartridges). Place mouse in the cage and expose to empty odor cartridge for 5 mins.
Pre-habituate mouse to a paper swab soaked in mineral oil in the odor cartridge. (5 mins).
Habituation phase. Expose mouse to mineral oil for three 50 secs trials with a 5 min interval between trials.
Detection phase. Expose mouse to lowest concentration of propionic acid for 50 secs and record time spent investigating the odor cartridge.
Repeat step 6 with the other concentrations of propionic acid, starting from lowest to highest.