Protocol Citation: sdwalto, Jeffrey Kordower, Bryan Killinger 2024. Odor Retention. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lyjoeqlx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 28, 2024
Last Modified: May 31, 2024
Protocol Integer ID: 95895
Keywords: ASAPCRN, odor threshold, mice behavior
Funders Acknowledgement:
Jeffrey Kordower
Grant ID: NIH R21 NS109871
Abstract
Odor retention test optimized for mice. This test assesses short term olfactory memory. Mice with olfactory impairments or deficits should score lower.
Guidelines
ODERANT PAIRS: limonene (diluted 1:10) and carvone (diluted 1:10); Amylacetate (1:100) and Anisol (1:100); and propyl acetate (1:10) and Pentanal (1:10).
Notes:
- Paper swabs soaked in a given odorant were used inside the cartridges.
- Investigation time defined as the duration of active sniffing with the nose placed less than 1 cm away from the cartridge.
Materials
- ODERANT PAIRS: limonene (diluted 1:10) and carvone (diluted 1:10); Amylacetate (1:100) and Anisol (1:100); and propyl acetate (1:10) and Pentanal (1:10).
- Paper swabs
- Mesh balls (tea balls)
Prepare and empty rodent cage with no bedding.
Prepare the odorants by diluting in mineral oil. (See guidelines)
Acclimate mouse to setup (empty cage with odor cartridges). Place mouse in the cage and expose to 2 empty odor cartridges on either side of the cage for 5 mins.
Expose mouse to the same unfamiliar odor in both cartridges for 5 mins. (Acquisition)
After 6, 16 or 30 mins post-acquisition, expose the mouse to a novel odorant and the familiar odorant from Step 4 and record the time spent investigating both the novel and familiar odorant for all trials. (Total investigation time capped at 1 min). (Recall)
The preferred odor was recorded and the preference was calculated based on the recall trial.