Nov 27, 2024
Version 2
OCT Embedding V.2
- 1Stanford University
- Human BioMolecular Atlas Program (HuBMAP) Method Development CommunityTech. support email: Jeff.spraggins@vanderbilt.edu
Protocol Citation: Joanna Bi 2024. OCT Embedding. protocols.io https://dx.doi.org/10.17504/protocols.io.3byl4qy6ovo5/v2Version created by Joanna Bi
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 17, 2024
Last Modified: November 27, 2024
Protocol Integer ID: 113017
Abstract
OCT embedding protocol
Sample tissue is collected fresh.
Squeeze a thin layer of OCT compound (Sakura Tissue-Tek O.C.T. Compound, ref 4583) into a disposable mold.
Place the sample tissue in the center of the mold, with the side to be cut oriented into the mold.
Squeeze more OCT compound over the top to full cover the tissue.
Lower the mold into liquid nitrogen, being careful to not fully submerge the mold. Hold in liquid nitrogen for a few seconds until fully frozen.
Place frozen mold in dry ice until stored in -80ºC