Reagent | Volume/amount | Final conc. (mM) | |
Sucrose 342.3 g/mol | 4.279g | 250 | |
2M KCL | 625ul | 25 | |
1M MgCl2 | 250ul | 5 | |
1MM Tris buffer | 500ul | 10 | |
Nuclease free water | 48.625ml | - | |
Total | 50ml |
Reagent | Volume | Final conc. | |
NIM1 | 4946ul | 1x | |
1mM DTT | 5ul | 1uM | |
100x protease inhibitor | 50ul | 1x | |
Total | 5mL |
Reagent | Volume | Final conc. | |
NIM2 | 4850ul | 1x | |
RNasin plus 40 U/ul | 50ul | 0.4U.ul | |
Superasin 20 U/ul | 50ul | 0.2U/ul | |
Triton X-100 10% | 50ul | 0.1% | |
Total | 5ml |
Reagent | Volume | Final conc. | |
1x PBS | 975ul | 1x | |
BSA powder | 0.05g | 5% | |
Rnasin protector | 25ul | 40U/ul | |
Total | 1ml |
Reagent | Volume | Final conc. | |
Lymphoprep | 1350ul | 90% | |
10x PBS | 150ul | 1x | |
Total | 1500ul |