Reagent | Vendor | Catalog Number | Storage | Notes | |
Minute Detergent-Free Single Nuclei Isolation Kit | Invent Biotech | NI-024 | Some reagents 4°C, some -20°C | ||
Protector RNase inhibitor | Millipore Sigma | 3335402001 | |||
Parse Evercode Nuclei Fixation | Parse Biosciences | 1 Parse Nuclei Fixation per 4 tissue samples | |||
MACS BSA Stock Solution | Miltenyi | 130-091-376 | 4°C | 10% BSA Solution | |
Molecular Biology Grade Water | Corning | 46-000-CI | Room Temperature | ||
Optional - RNAqueous Micro Total RNA Isolation Kit | ThermoFisher | AM1931 | 4°C |
Number of Samples | 1 | 2 | 3 | 4 | |
Nuclei Buffer (plus RNase inhibitor) | 750 | 1,500 | 2,250 | 3,000 | |
MACS BSA Stock Solution | 50 | 100 | 150 | 200 | |
Total (uL) | 800 | 1,600 | 2,400 | 3,200 |