All stock solutions should be filtered using a .22 um PVDF/PES filter system. All solutions except 50% Iodixanol solution are stable at 4c for at least 6 months.
Substance: Stock Conc.: Amount: Final conc. in working solution:
1.0616x Homogenization Buffer Stable Solution (200 mL)
Substance: Stock conc: Amount: Final conc. in working solution:
Sucrose 1 M 53.1 mL .2653 M
MgCl2 1 M 1.06 mL 5.31 mM
Tricine-KOH pH 7.8 .75 M 5.67 mL 21.2 mM
Substance: Stock conc: Amount: Final conc. in working solution:
Tricine KOH pH 7.8 .75 M 16 mL 120 mM
50% Iodixanol Solution (50 mL) **Remake Monthly for Stability
Substance: Stock conc: Amount: Final conc. in working solution:
Diluent Buffer - 8.3 mL -
Iodixanol 60% 41.7 mL 50%
1x Homogenization Buffer Unstable Solution for 4 reactions Prepare Fresh
Substance: Stock conc: Amount: Final conc. in working solution:
HB stable solution 1.0616X 7536 uL 1X
Spermidine 500 mM 8 uL .5 mM
Spermine 150 mM 8 uL .15 mM
cOmplete PI 100X 80 uL 1X
Ribolock 40U/uL 120 uL .6 U/uL
30% Iodixanol Solution per reaction Prepare Fresh
Substance: Stock conc: Amount: Final conc. in working solution:
50% Iodixanol Solution 50% 360 uL 30%
40% Iodixanol Solution per reaction Prepare Fresh
Substance: Stock conc: Amount: Final conc. in working solution:
50% Iodixanol Solution 50% 480 uL 40%
Wash Buffer 1 mL for 4 reactions Prepare Fresh
Substance: Stock conc: Amount: Final conc. in working solution:
Tris-HCl pH 7.4 1 M 10 uL 10 mM
Ribolock 40 U/uL 15 uL .6 U/uL