A | B | C | D | E | F | G | H | I | J | K | |
NLDM Solution 1 - 'Sugars' - 10X | |||||||||||
# | Compound | formula | class | Supplier | Supplier # | MW (avg) | Final_Conc_mmol/L | Conc_Factor | Conc mmol/L | mg for 50 mL stock solution | |
1 | sucrose | C12H22O11 | sugar | Sigma | S3929-1KG | 342.3 | 0.875 | 10 | 8.75 | 149.8 | |
2 | D-glucose | C6H12O6 | sugar | Sigma | G8270-1KG | 180.16 | 0.875 | 10 | 8.75 | 78.8 | |
3 | trehalose dihydrate | C12H22O11 x 2H2O | sugar | Santa Cruz Biotechnologies | sc-202368 | 378.33 | 0.875 | 10 | 8.75 | 165.5 | |
4 | myo-inositol | C6H12O6 | sugar | Sigma | I5125-50G | 180.16 | 0.875 | 10 | 8.75 | 78.8 | |
5 | xylose | C5H10O5 | sugar | Spectrum | XY110 | 150.13 | 0.875 | 10 | 8.75 | 65.7 | |
6 | D-mannitol | C6H14O6 | sugar | Sigma | M4125-500G | 182.17 | 0.875 | 10 | 8.75 | 79.7 | |
7 | n-acetyl glucosamine | C8H15NO6 | sugar | Sigma | A4106-25G | 221.21 | 0.875 | 10 | 8.75 | 96.8 | |
NLDM Solution 2 - 'Organic_Acids' - 10X | |||||||||||
# | Compound | formula | class | Supplier | Supplier # | MW (avg) | Final_Conc_mmol/L | Conc_Factor | Conc mmol/L | mg for 50 mL stock solution | |
8 | sodium-DL-lactate | C3H5NaO2 | organic acid | Sigma | 71720-5G | 112.06 | 0.525 | 10 | 5.25 | 29.4 | |
9 | DL-Malic acid | C4H6O5 | organic acid | Sigma | 240176-50G | 134.09 | 0.525 | 10 | 5.25 | 35.2 | |
10 | Citric acid | C6H8O7 | organic acid | Sigma | C4540-100G | 192.12 | 0.525 | 10 | 5.25 | 50.4 | |
11 | Succinic acid | C4H6O4 | organic acid | Sigma | S3674-100G | 118.09 | 0.525 | 10 | 5.25 | 31.0 | |
12 | sodium pyruvate | C3H3O3Na | organic acid | Sigma | P2256-25G | 110.04 | 0.525 | 10 | 5.25 | 28.9 | |
13 | alpha ketoglutaric acid | C5H6O5 | organic acid | Sigma | K1750-100G | 146.1 | 0.525 | 10 | 5.25 | 38.4 | |
14 | L-Citrulline | C6H13N3O3 | organic acid | Sigma | C7629-5G | 175.19 | 0.525 | 10 | 5.25 | 46.0 | |
NLDM Solution 3 - 'Amino_Acids' - 10X | |||||||||||
# | Compound | formula | class | Supplier | Supplier # | MW (avg) | Final_Conc_mmol/L | Conc_Factor | Conc mmol/L | mg for 50 mL stock solution | |
15 | L-Alanine | C3H7NO2 | amino acid | Sigma | A7627-100G | 89.09 | 0.175 | 10 | 1.75 | 7.8 | |
16 | L-Arginine | C6H14N4O2 | amino acid | Sigma | A8094-100G | 174.2 | 0.175 | 10 | 1.75 | 15.2 | |
17 | L-Asparagine | C4H8N2O3 | amino acid | Sigma | A0884-25G | 132.12 | 0.175 | 10 | 1.75 | 11.6 | |
18 | L-Aspartic acid | C4H7NO4 | amino acid | Sigma | A9256-100G | 133.1 | 0.175 | 10 | 1.75 | 11.6 | |
19 | L-Cysteine | C3H7NO2S | amino acid | Sigma | 30089-25G | 121.16 | 0.175 | 10 | 1.75 | 10.6 | |
20 | L-Glutamic acid | C5H9NO4 | amino acid | Sigma | 49449-100G | 147.13 | 0.175 | 10 | 1.75 | 12.9 | |
21 | L-Glutamine | C5H10N2O3 | amino acid | Sigma | G3126-100G | 146.14 | 0.175 | 10 | 1.75 | 12.8 | |
22 | Glycine | C2H5NO2 | amino acid | Sigma | 410225-50G | 75.07 | 0.175 | 10 | 1.75 | 6.6 | |
23 | L-Histidine | C6H9N3O2 | amino acid | Sigma | H8000-10G | 155.15 | 0.175 | 10 | 1.75 | 13.6 | |
24 | L-Isoleucine | C6H13NO2 | amino acid | Sigma | I2752-25G | 131.17 | 0.175 | 10 | 1.75 | 11.5 | |
25 | L-Leucine | C6H13NO2 | amino acid | Sigma | L8912-25G | 131.17 | 0.175 | 10 | 1.75 | 11.5 | |
26 | L-Lysine | C6H14N2O2 | amino acid | Sigma | L5501-25G | 146.19 | 0.175 | 10 | 1.75 | 12.8 | |
27 | L- Methionine | C5H11NO2S | amino acid | Sigma | M5308-25G | 149.21 | 0.175 | 10 | 1.75 | 13.1 | |
28 | L-Phenylalanine | C9H11NO2 | amino acid | Sigma | P5482-25G | 165.19 | 0.175 | 10 | 1.75 | 14.5 | |
29 | L-Proline | C5H9NO2 | amino acid | Sigma | P5607-25G | 115.13 | 0.175 | 10 | 1.75 | 10.1 | |
30 | L-Serine | C3H7NO3 | amino acid | Sigma | S4311-25G | 105.09 | 0.175 | 10 | 1.75 | 9.2 | |
31 | L-Threonine | C4H9NO3 | amino acid | Sigma | T8441-25G | 119.12 | 0.175 | 10 | 1.75 | 10.4 | |
32 | L-Tryptophan | C11H12N2O2 | amino acid | Sigma | T8941-25G | 204.23 | 0.175 | 10 | 1.75 | 17.9 | |
33 | L-Tyrosine | C9H11NO3 | amino acid | Sigma | T8566-25G | 181.19 | 0.175 | 10 | 1.75 | 15.9 | |
34 | L-Valine | C5H11NO2 | amino acid | Sigma | 94619-25G | 117.15 | 0.175 | 10 | 1.75 | 10.3 | |
NOTE: Dissolve all amino acids in 4 mM HCl. Sonicate for 30 minutes to dissolve all amino acids | |||||||||||
NLDM Solution 4 - 'Nucleobases/Nucleosides' - 50X | |||||||||||
# | Compound | formula | class | Supplier | Supplier # | MW (avg) | Final_Conc_mmol/L | Conc_Factor | Conc mmol/L | mg for 50 mL stock solution | |
35 | 5-methyluridine | C10H14N2O6 | nucleoside | Sigma | 535893-25G | 258.23 | 0.0175 | 50 | 0.875 | 11.3 | |
36 | Adenine | C5H5N5 | nucleobase | Sigma | A8626-5G | 135.13 | 0.0175 | 50 | 0.875 | 5.9 | |
37 | Adenosine | C10H13N5O4 | nucleoside | Sigma | A4036-5G | 267.24 | 0.0175 | 50 | 0.875 | 11.7 | |
38 | Cytidine | C9H13N3O5 | nucleoside | Sigma | C4654-5G | 243.22 | 0.0175 | 50 | 0.875 | 10.6 | |
39 | Cytosine | C4H5N3O | nucleobase | Sigma | C3506-1G | 111.1 | 0.0175 | 50 | 0.875 | 4.9 | |
40 | Guanine | C5H5N5O | nucleobase | Sigma | G11950-100G | 151.13 | 0.0175 | 50 | 0.875 | 6.6 | |
41 | Guanosine | C10H13N5O5 | nucleoside | Sigma | G6264-5G | 283.24 | 0.0175 | 50 | 0.875 | 12.4 | |
42 | Hypoxanthine | C5H4N4O | nucleobase | Sigma | H9636-5G | 136.11 | 0.0175 | 50 | 0.875 | 6.0 | |
43 | Inosine | C10H12N4O5 | nucleoside | Sigma | I4125-100G | 268.23 | 0.0175 | 50 | 0.875 | 11.7 | |
44 | Thymine | C5H6N2O2 | nucleobase | Sigma | T0895-5G | 126.11 | 0.0175 | 50 | 0.875 | 5.5 | |
45 | Uracil | C4H4N2O2 | nucleobase | Sigma | U1128-25G | 112.09 | 0.0175 | 50 | 0.875 | 4.9 | |
46 | Uridine | C9H12N2O6 | nucleoside | Sigma | U3003-5G | 244.2 | 0.0175 | 50 | 0.875 | 10.7 | |
47 | Xanthine | C5H4N4O2 | nucleobase | Sigma | X0626-5G | 152.11 | 0.0175 | 50 | 0.875 | 6.7 | |
48 | Xanthosine dihydrate | C10H12N4O6 | nucleobase | Sigma | X0750-5G | 320.26 | 0.0175 | 50 | 0.875 | 14.0 | |
NOTE: Dissolve all nucleobases and nucelosides in 0.08M NaOH | |||||||||||
NLDM Solution 5 - 'Other_Cpds' - 50X | |||||||||||
# | Compound | formula | class | Supplier | Supplier # | MW (avg) | Final_Conc_mmol/L | Conc_Factor | Conc mmol/L | mg for 50 mL stock solution | |
49 | gamma-Aminobutyric acid | C4H9NO2 | other | Sigma | A2129-25G | 103.12 | 0.0175 | 50 | 0.875 | 4.5 | |
50 | Ectoine | C6H10N2O2 | other | Sigma | 81619-1G-F | 142.16 | 0.0175 | 50 | 0.875 | 6.2 | |
51 | Betaine | C5H11NO2 | amino acid deriv | Sigma | 61962-50G | 117.15 | 0.0175 | 50 | 0.875 | 5.1 | |
52 | N-acetyl-L-Glutamic acid | C7H11NO5 | amino acid deriv | Sigma | 855642-25G | 189.17 | 0.0175 | 50 | 0.875 | 8.3 | |
53 | Nicotinamide | C6H6N2O | vitamin | Sigma | 72340-100G | 122.12 | 0.0175 | 50 | 0.875 | 5.3 | |
54 | Ornithine HCl | C5H12N2O2 | amino acid deriv | Sigma | O2375-5G | 168.62 | 0.0175 | 50 | 0.875 | 7.4 | |
55 | Shikimic acid | C7H10O5 | other | Sigma | S5375-1G | 174.15 | 0.0175 | 50 | 0.875 | 7.6 | |
56 | spermidine | C7H19N3 | other | Sigma | S0266-1G | 145.25 | 0.0175 | 50 | 0.875 | 6.4 | |
57 | taurine | C2H7NO3S | other | Sigma | T0625-10G | 125.15 | 0.0175 | 50 | 0.875 | 5.5 | |
58 | Trigonelline HCl | C7H7NO2 x HCl | other | Sigma | T5509-5G | 173.6 | 0.0175 | 50 | 0.875 | 7.6 | |
59 | L-Carnitine HCl | C7H16NO3 x HCl | other | Sigma | C0283-1G | 197.66 | 0.0175 | 50 | 0.875 | 8.6 | |
60 | Choline Cl | C5H14NO | other | Sigma | C7017-5G | 139.62 | 0.0175 | 50 | 0.875 | 6.1 | |
61 | N-acetyl-L-Glutamine | C7H12N2O4 | amino acid deriv | Sigma | A9125-25G | 188.18 | 0.0175 | 50 | 0.875 | 8.2 | |
62 | n-acetyl-L-lysine | C8H16N2O3 | amino acid deriv | Sigma | A4021-1G | 188.22 | 0.0175 | 50 | 0.875 | 8.2 | |
63 | n-acetylmuramic acid | C11H19NO8 | other | Sigma | A3007-500MG | 293.27 | 0.0175 | 50 | 0.875 | 12.8 | |
64 | sn-glycero-3-phosphocholine | C8H20NO6P | other | Cayman | 20736 | 257.22 | 0.0175 | 50 | 0.875 | 11.3 |
A | B | C | D | E | F | G | H | I | J | K | |
NLDM Salt solution 1 - 'MgSO4' - 50X | |||||||||||
# | Compound | formula | class | Supplier | Supplier # | MW (avg) | Final_Conc_mmol/L | Conc_Factor | Conc mmol/L | mg/50ml | |
S1 | Magnesium sulfate heptahydrate | MgSO4 * 7H2O | Salt | Sigma | M2773-500G | 246.47 | 0.812 | 50 | 40.6 | 500.3 | |
NLDM Salt solution 2 - 'NH4Cl' - 50X | |||||||||||
# | Compound | formula | class | Supplier | Supplier # | MW (avg) | Final_Conc_mmol/L | Conc_Factor | Conc mmol/L | mg/50ml | |
S2 | Ammonium chloride | NH4Cl | Salt | Sigma | A9434-500G | 53.49 | 1 | 50 | 50 | 133.7 | |
NLDM Salt solution 3 - 'CaCl2' - 50X | |||||||||||
# | Compound | formula | class | Supplier | Supplier # | MW (avg) | Final_Conc_mmol/L | Conc_Factor | Conc mmol/L | mg/50ml | |
S3 | Calcium chloride dihydrate | CaCl2 * 2 H2O | Salt | Sigma | C7902-500G | 147.01 | 0.68 | 50 | 34 | 249.9 | |
NLDM Salt solution 4 - 'PO4' - 50X | |||||||||||
# | Compound | formula | class | Supplier | Supplier # | MW (avg) | Final_Conc_mmol/L | Conc_Factor | Conc mmol/L | mg/50ml | |
S4 | Sodium phosphate monobasic | NaH2PO4 | Salt | Sigma | S0751-500G | 119.98 | 1.665 | 50 | 83.25 | 499.4 | |
S5 | Potassium phosphate dibasic | K2HPO4 | Salt | Sigma | P3786-500G | 174.18 | 3.33 | 50 | 166.5 | 1450.0 |
A | |
Medium components | |
NLDM Solution 1 - 'Sugars' - 10X | |
NLDM Solution 2 - 'Organic_Acids' - 10X | |
NLDM Solution 3 - 'Amino_Acids' - 10X | |
NLDM Solution 4 - 'Nucleobases/Nucleosides' - 50X | |
NLDM Solution 5 - 'Other_Cpds' - 50X | |
NLDM Salt solution 1 - 'MgSO4' - 50X | |
NLDM Salt solution 2 - 'NH4Cl' - 50X | |
NLDM Salt solution 3 - 'CaCl2' - 50X | |
NLDM Salt solution 4 - 'PO4' - 50X | |
Vitamin solution - 100X | |
Trace Mineral solution - 100X |
A | B | |
Volume (mL) | ||
NLDM Solution 1 - 'Sugars' - 10X | 50 | |
NLDM Solution 2 - 'Organic_Acids' - 10X | 50 | |
NLDM Solution 3 - 'Amino_Acids' - 10X | 50 | |
NLDM Solution 4 - 'Nucleobases/Nucleosides' - 50X | 10 | |
NLDM Solution 5 - 'Other_Cpds' - 50X | 10 | |
NLDM Salt solution 1 - 'MgSO4' - 50X | 10 | |
NLDM Salt solution 2 - 'NH4Cl' - 50X | 10 | |
NLDM Salt solution 3 - 'CaCl2' - 50X | 10 | |
NLDM Salt solution 4 - 'PO4' - 50X | 10 | |
Vitamin solution - 100X | 5 | |
Trace Mineral solution - 100X | 5 | |
Milli-Q Water | 280 | |
TOTAL | 500 |