Mar 19, 2022
Nissl (Cresyl Violet) staining
- Christiana.bjorkli1,
- Karoline Hovde1,
- Bruno Monterotti1
- 1The Norwegian University of Science and Technology
Protocol Citation: Christiana.bjorkli, Karoline Hovde, Bruno Monterotti 2022. Nissl (Cresyl Violet) staining. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gpbzxplzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: March 19, 2022
Last Modified: March 19, 2022
Protocol Integer ID: 59637
Abstract
Nissl (Cresyl Violet) staining protocol
Dehydrate sections – 10 dips in each: 50, 70, 80, 90, 100, 100, and 100% ethanol
Leave in xylene for 2min
2m
Rehydrate sections – 10 dips in each: 100, 100, 100, 90, 80, 70, and 50% ethanol
Quick wash in running water
Leave sections in Cresyl Violet for 3min on shake
3m
Leave sections in Citric Acid for 1min
1m
Dip in running water 3x
Dip in Citric Acid 3x
Dip in running water 3x
Leave sections in Cresyl Violet for 2min on shaker
2m
Repeat step 6
Dehydrate sections – 10 dips in each: 50, 70, 80, 90, 100, 100, and 100% ethanol
Leave in Xylene for at least 5min
5m
Coverslip with Xylene + Entellan