Mar 27, 2025
NIS-Elements JOB for DNA-Paint Acquisition at Serial Z-Planes
- Robert Tetley1,
- Andrew Vaughan2,
- Cecilia Zaza2
- 1Nikon Healthcare UK;
- 2University College London
- Nikon Healthcare UK, Branch of Nikon Europe
Protocol Citation: Robert Tetley, Andrew Vaughan, Cecilia Zaza 2025. NIS-Elements JOB for DNA-Paint Acquisition at Serial Z-Planes. protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvj9p4nlk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 30, 2024
Last Modified: March 27, 2025
Protocol Integer ID: 117415
Disclaimer
Caution is advised when using the JOB, as stage movement is unsupervised. Setting z-drive limits is strongly recommended.
Abstract
This JOB was created in NIS-Elements version 5.42.06, and is designed to acquire DNA-Paint images at multiple serial Z-planes. At each Z-plane, a user-defined number of images is captured. Focus stability is maintained at each plane through the use of Nikon's Perfect Focus System (PFS).
Guidelines
This JOB was created in NIS-Elements version 5.42.06.
Materials
MultiZ_DNAPaint.bin15KB JOB definition for DNA-Paint and Serial Z-Planes - to be imported into the NIS-Elements JOBS Explorer:Run the JOB from the JOBS Explorer
Run the JOB from the JOBS Explorer
Download the attached JOB definition (see the Materials tab) and import the file into the JOBS Explorer
Click on the name of the JOB definition in the list and click the green Play icon
In the JOBS Wizard, first define an Alternative Storage Location where images will be saved
Select the Optical Configurations/Experiment Setups that define your Capture Definition
Select the Z-stack parameters for image capture, including z-range, z-step size and z-device.
In the Time Lapse parameters, define the number of images to be captured at each z-plane (by selecting "Number of Loops"). For fastest imaging, select No Delay as the Acquisition Period.
Once all Wizard sections are complete, click Run
When the JOB starts running, you will first be prompted to enter a file name for your images. Once this is defined, click OK.
The JOB will then begin acquiring images at each z-plane after automatically defining PFS offsets for each plane. If you would like to finish acquiring before images have been captured at every z-plane, click Finish. If you have made an error during setup and wish to discard all image data from the experiment, click Abort.