Feb 07, 2024

Public workspaceNest Building Test

DOI
dx.doi.org/10.17504/protocols.io.e6nvwdr42lmk/v1
  • Valina L. Dawson1,2,3,4,5,6,
  • Ted Dawson1,2,3,4,5,6,7,
  • Hanseok Ko1,2,3
  • 1Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;
  • 2Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;
  • 3Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA;
  • 4Adrienne Helis Malvin Medical Research Foundation, New Orleans, LA 70130-2685, USA;
  • 5Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205 USA;
  • 6Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;
  • 7Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA
Eileen Ruth Torres
Open access
DOI: dx.doi.org/10.17504/protocols.io.e6nvwdr42lmk/v1
Protocol Citation: Valina L. Dawson, Ted Dawson, Hanseok Ko 2024. Nest Building Test. protocols.io https://dx.doi.org/10.17504/protocols.io.e6nvwdr42lmk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 07, 2024
Last Modified: May 31, 2024
Protocol Integer ID: 94862
Keywords: ASAPCRN, behavior, fine motor skills
Disclaimer
The protocols.io team notes that research involving animals and humans must be conducted according to internationally-accepted standards and should always have prior approval from an Institutional Ethics Committee or Board.
Abstract
Used to assess nigrostriatal sensorimotor function in mice. Based on the Deacon 2006 protocol.
Materials
Cotton nestlets
Place 2.5 g nestlet into feeder of each cage.
Individually house mice must then pull the cotton nestlet from the feeder by rearing and useing complext fine motor skills.
Scores based on the amount of unused nestlet material were determined 16 hours after the nestlet was placed. Further details on scoring can be seen in Deacon 2006.
Protocol references
Deacon, Robert MJ. "Assessing nest building in mice." Nature protocols 1.3 (2006): 1117-1119.