pGST2-GST-TEV-NDP52 (Addgene ID: 187828)
pGST2-GST-TEV-OPTN S177D S473D (Addgene ID: 187827)
E. coli Rosetta pLysS cells
LB medium with antibiotics: 50 μl/ml ampicillin and 34 μl/ml chloramphenicol
IPTG (Isopropyl-β-D-thiogalactopyranosid)
Complete inhibitor EDTA free Roche
Wash Buffer I (low salt):
Wash Buffer II (high salt):
Glutathione Sepharose 4B (Cytiva)
Superose 6 increase 10/300 column (Cytiva)