May 13, 2023

Public workspaceNCEM Drop - Tissue Dounce Homogenisation (TM-014)

DOI
dx.doi.org/10.17504/protocols.io.eq2ly7odwlx9/v1
  • 1CSIRO ACDP
sandra.crameri
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DOI: dx.doi.org/10.17504/protocols.io.eq2ly7odwlx9/v1
Protocol Citation: sandra.crameri 2023. NCEM Drop - Tissue Dounce Homogenisation (TM-014). protocols.io https://dx.doi.org/10.17504/protocols.io.eq2ly7odwlx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 13, 2023
Last Modified: May 13, 2023
Protocol Integer ID: 81823
Abstract
tissue dounce homogenisation
Protocol materials
Reagentnano-WCatalog ##2018-5ML
Step 9
HEADER
HEADER
SAN:
SPEC No:
OPERATOR:

Dounce Homogenisation
Dounce Homogenisation
This method is often used for scabs, warts, or chunks of tissue such as liver. Place ~ 5mm x 5mm piece of scab or tissue into glass homogeniser tube.

Insert teflon plunger into cordless drill, set drill on max speed.
Add equal volume TC water to tube, homogenise(20 strokes) inside Class II BSC cabinet.
Clarify resultant solution at Centrifigation13000 rpm in Eppendorf centrifuge for Duration00:01:00


1m
Stand for Duration00:05:00 to permit viruses to diffuse back into solution, from the debris, and provide an interface for the sampling of membrane associated viruses. Use supernatant for sample below.

5m
Conventional
Conventional
11m
11m
Adsorb Amount10 µL sample to grid Duration00:10:00 , inspect to ensure sample does not dry out.

10m
Drain excess sample from grid using filter paper, leave wet.
Stain Reagentnano-WCatalog ##2018-5ML Duration00:01:00

1m
Drain & dry using filter paper