May 13, 2023

Public workspaceNCEM Drop - Paramyxo confirmation, generation of RNP (TM-014)

DOI
dx.doi.org/10.17504/protocols.io.x54v9dq8pg3e/v1
  • 1CSIRO ACDP
sandra.crameri
Icon indicating open access to content
QR code linking to this content
DOI: dx.doi.org/10.17504/protocols.io.x54v9dq8pg3e/v1
Protocol Citation: sandra.crameri 2023. NCEM Drop - Paramyxo confirmation, generation of RNP (TM-014). protocols.io https://dx.doi.org/10.17504/protocols.io.x54v9dq8pg3e/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 13, 2023
Last Modified: May 13, 2023
Protocol Integer ID: 81822
Abstract
Generate RNP to confirm Paramyxoviurs
Protocol materials
Reagentnano-WCatalog ##2018-5ML
Step 6
ReagentDeoxycholic acid sodium saltMerck MilliporeSigma (Sigma-Aldrich)Catalog #D-6750
Step 2
HEADER
HEADER
SAN:
SPEC No:
OPERATOR:

Generation of RNP
Generation of RNP
Prepare Concentration0.5 Mass / % volume ReagentDeoxycholic acid sodium saltMerck MilliporeSigma (Sigma-Aldrich)Catalog #D-6750 in TC Water.

Mix 1:1 with the sample for Duration00:05:00

5m
Conventional
Conventional
11m
11m
Adsorb Amount10 µL sample to grid Duration00:10:00 , inspect to ensure sample does not dry out.

10m
Drain excess sample from grid using filter paper, leave wet.
Stain Reagentnano-WCatalog ##2018-5ML Duration00:01:00

1m
Drain & dry using filter paper