May 13, 2023

Public workspaceNCEM Drop Agar (TM-014)

DOI
dx.doi.org/10.17504/protocols.io.rm7vzb4n5vx1/v1
  • 1CSIRO ACDP
sandra.crameri
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DOI: dx.doi.org/10.17504/protocols.io.rm7vzb4n5vx1/v1
Protocol Citation: sandra.crameri 2023. NCEM Drop Agar (TM-014). protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzb4n5vx1/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 13, 2023
Last Modified: May 13, 2023
Protocol Integer ID: 81826
Abstract
drop agar
Protocol materials
ReagentAgaroseCatalog #A5304
Step 2
Reagentnano-WCatalog ##2018-5ML
Step 5
HEADER
HEADER
SAN:
SPEC No:
OPERATOR:

Agar preparation
Agar preparation
Make Concentration2 Mass / % volume ReagentAgaroseContributed by usersCatalog #A5304 in TC Water. Microwave to dissolve, pour intoAmount3 mL of the solution into Amount5 mL petri dishes and place in fridge to solidify.

Cut 1 x1 cm squares out of agar with scalpel, lift out onto filter paper
Adsorption & Staining
Adsorption & Staining
1m
1m
Place Amount5 µL sample on surface of agar square. Immediately float carbon coated formvar filmed grid face down on the droplet and leave until the droplet diffuses into the agar.

Stain Reagentnano-WCatalog ##2018-5ML Duration00:01:00

1m
Drain & dry using filter paper