A | B | |
Reagent | Volume (µl) | |
Ultra II End-prep reaction buffer | 1.75 | |
Ultra II End-prep enzyme mix | 0.75 |
A | B | |
Reagent | Volume (µl) | |
End-prepped DNA | 3.75 | |
Native barcode | 1.25 | |
Blunt/TA Ligase Master Mix | 5 |
A | B | |
Native Adapter (NA) | 5uL | |
NEBNext Quick LIgation Reaction Buffer (5x) | 10uL | |
NEB Quick T4 Ligase | 5uL |
A | B | |
Reagent | Volume (μL) | |
DNA library | 12 | |
Sequencing buffer (SB) | 37.5 | |
Library beads (LIB) | 25.5 |