The Kit 12 chemistry runs at 30°C on nanopore sequencing devices. This is several degrees cooler than other chemistries. While the protocol was initially developed on GridION and PromethION, we also support its use on MinION Mk1C, as the MinION Mk1C device's temperature control allows the flow cell to be maintained at 30°C for the duration of the run. However, we cannot guarantee the same level of temperature control on the MinION Mk1B. Therefore, if you are running Kit 12 chemistry on the MinION Mk1B, ensure that the ambient temperature does not exceed 23°C.
Adapter Mix II H (AMII H) used in this kit and protocol is not interchangeable with other sequencing adapters. This kit and protocol is only compatible with Adapter Mix II H (AMII H). If more sequencing adapter is required, theNative Barcoding Expansion (EXP-NBD112) is available for additional reagents. Adapter Mix II H (AMII H) is a new sequencing adapter for Kit 12 chemistry and is loaded with an updated sequencing enzyme with improved accuracies and has a higher capture rate to lower flow cell loading amounts. It also contains the fuel fix technology to enable longer runs without the need for fuel addition during a run. Therefore, sequencing adapters from other kits are not compatible with this kit or protocol.