A | B | C | |
Reagent | Volume | Color | |
49~100fmol sample DNA + DI Water | 50μl | ||
End Repair&A-Tailing Buffer (KAPA) | 7μl | Purple | |
End Repair&A-Tailing Enzyme Mix (KAPA) | 3μl | Purple | |
Total | 60μl |
A | B | C | |
Reagent | Volume | Color | |
End repaired & A-tailing product | 60μl | ||
Adapter Mix (AMX) | 5μl | Green | |
DI Water | 5μl | ||
Ligation Buffer (KAPA) | 30μl | Yellow | |
NEBNext Quick T4 DNA Ligase (KAPA) | 10μl | Yellow | |
Total | 110μl |
A | B | C | |
Reagent | Volume | Color | |
Ligation product | 110μl | ||
Agencourt AMPure XP beads | 44μl | Stored in 4°C | |
Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) | 250μl (twice) | Orange & Grey | |
Elution Buffer from the Oxford Nanopore kit (EB) | 14μl | Black |
A | B | C | |
Reagent | Volume | Color | |
Flush Tether (FLT) | 30μl | Purple | |
Flush Buffer (FB) | New one | Blue | |
Total | 1.2ml |
A | B | C | |
Reagent | Volume | Color | |
Sequencing Buffer (SQB) | 37.5μl | Red | |
Loading Beads (LB),mixed immediately before use | 25.5μl | Pink | |
DNA library | 12μl | ||
Total | 75μl |
A | B | C | |
Component | Volume | Color | |
Wash Mix (WMX) | 2μl | Brown | |
Wash Diluent (DIL) | 398μl | Brown | |
Total | 400μl |