Reagent | Volume | |
DNA | 50 µL | |
KAPA HyperPrep End Repair & A-tailing Buffer | 7 µL | |
KAPA HyperPrep End Repair & A-tailing Enzyme | 3 µL |
Reagent | Volume | |
End-prep product | 60 µL | |
Ligation adapter | 5 µL | |
ddH2O | 5 µL | |
Ligation Buffer | 30 µL | |
Ligase | 10 µL |
Reagent | Volume | |
Sequencing Buffer (SB) | 37.5 µL | |
Library Solution (LIS) | 25.5 µL |
Reagent | Volume | |
Wash Mix (WMX) | 1 µL | |
Wash Diluent (DIL) | 199 µL |