Component | Volume | x(n+2) | LOT | |
Superscript III | 0.5 µL | |||
TUNI 12.4/13 (10uM) | 1 uL | |||
Nuclease Free Water | 1.75 µL | |||
Reaction Mix x2 | 6.25 µL | |||
Total | 9.5 µL |
Component | Volume | x(x+2) | LOT | |
Superscript III | 0.5 µL | |||
Nuclease Free Water | 1.75 µL | |||
Reaction Mix x2 | 6.25 µL | |||
TUNI 12/13 (10uM) | 1 uL | |||
Total | 9.5 µL |
Step | Temperature | Time | Cycles | |
RT step | 55 | 30 minutes | 1 | |
Denaturation | 94 | 2 minutes | 1 | |
Denaturation | 94 | 30 seconds | 5 | |
Annealing | 45 | 30 seconds | 5 | |
Extension | 68 | 3 minutes | 5 | |
Denaturation | 94 | 30 seconds | 31 | |
Annealing | 57 | 30 seconds | 31 | |
Extension | 68 | 3 minutes | 31 | |
Final Extension | 68 | 3 minutes | 1 | |
Hold | 4 | - | - |
Reagent | Volume | |
SBII | 37.5 uL | |
LBII | 25.5 uL | |
DNA library | 12 uL | |
Total | 75 uL |