A | B | |
Sterile water | 7 ul | |
10X T4 Polynucleotide Kinase Reaction Buffer (with 1 mM ATP) | 2 ul | |
T4 Polynucleotide Kinase (10U/ul) | 1 ul | |
PCR product (x10 samples) | 1 ul x 10 samples = 10 ul |
A | B | |
5' Phosphorylated PCR product (50ng/ul) | 10 ul | |
Sterile water | 7 ul | |
10X T4 DNA Ligase Buffer (with 1 mM ATP) | 2 ul | |
Adapter | 0.1 ul | |
T4 ligase | 1 ul (300U or 2.5U in Weiss Unit) |