Heavily contaminated cell lines or from labs with unknown mycoplasma testing procedures:
MRA treatment (Fluoroquinolone class antibiotic)
Reagent: Mycoplasma Removal Agent, MP Biomedicals, cat #093050044 - 5 ml
(Stored at room temperature)
1. Split cells very thinly (<20% confluent) in regular tissue culture media
2. Add 10ul MRA per mL (up to 1mL total in a 6 wells plate)
3. Refresh media and MRA every 2 days over the course of 8 days (4 rounds of treatment)
4. Test for mycoplasma (See Mycoplasma Testing Protocol)
***If cells are negative, they can be used normally (label cryo stocks as G1)
***If cells are borderline, move to Step 4 – Plasmocin treatment
***If cells are positive, go directly to Step 5 – BM-Cyclin treatment