We have developed a workflow to assess the spatial organization of liver tissue with regard to its cellular and metabolic heterogeneity at tissue and single cell level resolution - by performing multimodal imaging on consecutive tissue sections using H&E staining, DESI, (H2O)n-GCIB-SIMS, C60-SIMS, and RNAscope.
This document provides the collection of protocols within the multimodal imaging workflow implemented by Columbia University/Pennsylvania State University Transformative Technology Development Center as a part of Human Biomolecular Atlas Program (HuBMAP, NIH).