Nov 03, 2022
MTT assay on 96 well plate
- 1Institute of Tropical Disease, Airlangga University
Protocol Citation: Adita Ayu Permanasari 2022. MTT assay on 96 well plate. protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvr4x5pgmk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 06, 2022
Last Modified: November 03, 2022
Protocol Integer ID: 69610
Abstract
MTT assay to measure viability cells
Prepare sample in DMSO
Observe cell on the plate, make a note about cells condition
Experiment by make a serial dilution sample in U-Type, suspend 40 times
Remove old medium from cells
Put 100 μl/well sample and DMSO, incubate 48 h, 37 oC
Observe condition cells after 48 h incubation
Checked toxicity under microscope, take photo if there are morphology changing
Prepare MTT 10%
Remove medium from 96 well plate
Put 150 μl/well by multichannel pipet, incubate 4 h, 37 oC
Remove MTT solution from well plate
Put 100 μl/well DMSO 100% for dissolve
Check absorbance at 560 nm and 750 nm , shaker 0.5 min before read absorbance