Apr 21, 2022
Version 9
Mouse Whole Cell Tissue Processing for 10x Genomics Platform V.9
- Allen Institute for Brain Science1
- 1Allen Institute
Protocol Citation: Allen Institute for Brain Science 2022. Mouse Whole Cell Tissue Processing for 10x Genomics Platform. protocols.io https://dx.doi.org/10.17504/protocols.io.q26g7b52klwz/v9Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: April 21, 2022
Last Modified: April 21, 2022
Protocol Integer ID: 61183
Keywords: 10x, genomics, whole cell, transcriptomic, isolate, extraction, processing, preparation, cell sorting, cell suspension, PF0307,
Abstract
This protocol describes how to isolate tissue from various ROI’s of the mouse brain followed by preparation of a highly concentrated single cell suspension for transcriptomic profiling on 10x Genomics platform.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
