SNpC Striatum Intraventrical Entorhinal Cortex
M/L: 1.2 M/L: 2.0 M/L: 1.0 M/L: -3.9
A/P: -3.2 A/P: 0.5 A/P: 0.3 A/P: -3.52
D/V: -4.6 D/V: -3.2 D/V: -2.7 D/V: -4.2
For pain management we use Slow Release (SR) Buprenorphine. Firstly, and very importantly, buprenorphine SR CANNOT be diluted. The drug is specifically formulated so it can have “sustained release” action and dilution will cause the drug to be essentially non-efficacious. Here are some tips for handling the relatively viscous drug:
Recommend warming the Buprenorphine SR-LAB vial in a incubator to around 1-2 degrees below animal body temperature. Warming it before drawing it into a syringe greatly reduces the polymer matrix viscosity
Use a larger needle (e.g. 17g) to insert to the vial with drugs to avoid pressure resistance
Use a 1 cc syringes featuring a low dead-space plunger with precise 0.01 ml graduation markings
In terms of concentration, the 0.5 mg/mL concentration as recommended by ARP is the best for calculating the appropriate doses. For ~25g mouse, we inject 50uL of the drug.
If using anal heating probe and pad, set probe to mouse internal temperature of 35deg C.