Protocol Citation: Sabina Marciano, Roberta Marongiu 2023. Mouse Ovariectomy. protocols.io https://dx.doi.org/10.17504/protocols.io.14egn338zl5d/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 14, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 86451
Keywords: ASAPCRN, Ovariectory
Abstract
This protocol outlines the steps to perform an Ovariectomy in a mouse
Anesthetize the mouse with Ketamine ( 100 µL / 100 g )
Apply eye ointment
Shave and disinfect the area below the ribs
Disinfect the surgical tools
Place the mouse on the side
Align 2 tweezers with the arms/legs and use the crossing point as a reference for the
incision
Perform a small incision and pull out the fat/ovary
Clamp right below and above the ovary
Remove the ovary
Suture both the inner and out layers
Disinfect the suture with antibiotic ointment