Using clean forceps (RNAse-away before first use, etOH between samples) remove filter from cryovial.
Keeping the filter over a fresh petri-dish half, cut small pieces of filter with clean dissecting scissors (RNase-away before first use, etOH between samples).
Transfer filter pieces to a bead tube with RLT+BME buffer.
Push the filter pieces down into the beads and buffer so that all filter surfaces remain submerged in the buffer.
Label the bead tube and put the remaining sample back on dry ice or directly back into the –80 freezer.
continue for all filters extracting from that day (max 24, recommend 10 or 12 at the beginning -- 10 is good so all samples can go on one bioanalyzer chip )