Place the DNeasy Mini spin column in a new 2 mL collection tube, add 500 μL Buffer AW2, and centrifuge for 5 min at 17,000 g to dry the DNeasy membrane. Discard flow-through and collection tube. Note 5: Following the centrifugation step, carefully remove the DNeasy Mini spin column, so it does not come into contact with the flow-through. This will result in a carryover of ethanol. If the carryover of ethanol occurs, empty the collection tube and reuse it in another centrifugation for 5 min at 17,000 g. Drying the DNeasy Mini spin column membrane is essential as residual ethanol could interfere with subsequent steps. This centrifugation ensures that no residual ethanol will be carried over during the following elution.