Compared to a classic one-step growth assay, this protocol uses multiple MOPs (virion:host ratios), miniaturization, and flow cytometry to increase efficiency. Briefly, viruses and host cells (algae) are mixed at a high concentration and allowed to adsorb, infections are synchronized by 1:1000 dilution, diluted suspensions are aliquoted, and independent aliquots are sampled every 2h. The samples are then measured with flow cytometry, and non-linear statistical models used to estimate the adsorption constant, lysis time, burst size, and depolarization probability. See the associated manuscript for a full description and schematic figures, as well as the statistical model.