Jun 01, 2023

Public workspaceModified Leeming and Notman Agar

DOI
dx.doi.org/10.17504/protocols.io.kxygxm3ywl8j/v1
  • 1Duke Universtiy
Amy Gladfelter
Icon indicating open access to content
QR code linking to this content
DOI: dx.doi.org/10.17504/protocols.io.kxygxm3ywl8j/v1
Protocol Citation: Amy Gladfelter 2023. Modified Leeming and Notman Agar. protocols.io https://dx.doi.org/10.17504/protocols.io.kxygxm3ywl8j/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 03, 2018
Last Modified: June 01, 2023
Protocol Integer ID: 17456
Abstract
Media recipe for growth of Malassezia species.
Attachments
Icon for Malassezia media_1.pdf
Malassezia media_1.p...
33KB
bacteriological peptone (Oxoid) Amount10 g

yeast extract Amount2 g

desiccated ox bile (Oxoid) Amount8 g

glucose Amount10 g

glycerol monostearate Amount0.5 g

agar Amount15 g

glycerol, 50% Amount20 mL

tween 60 Amount5 mL

olive oil Amount20 mL

dH2O Amount955 mL

Sterilize by autoclaving.