Protocol Citation: 孟筠 陳 2023. Modified beetle rearing medium. protocols.io https://protocols.io/view/modified-beetle-rearing-medium-cmutu6wn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 17, 2023
Last Modified: September 13, 2023
Protocol Integer ID: 75379
Funders Acknowledgement:
Yin-Tse Huang
Abstract
Materials
1. 9~10 Glass tubes (20*3.5cm)with plug for only one side (opening of the other side )
2. a bundle of tin foil
3. big plastic box for mixing the ingredients
Start making the medium!
Start making the medium!
First, Add the dry ingredients
| A | B | |
| Chloramphenicol | 0.47g | |
| Wesson's salt | 1.33g | |
| Yeast | 6.65g | |
| Casein | 6.65g | |
| Starch | 6.65g | |
| Surcose | 13.3g | |
| Agar | 26.6g | |
| Liquidambar Sawdust | 130g |
Note
*The Sawdust need to be ground before using
Second, add the wet ingredients
| A | B | |
| wheat germ oil | 2.5ml | |
| 95% ethanol | 5ml | |
| water | 500ml(still need to be test) | |
a little bit too wet for adding 565ml of water
MIx all of the ingredients in the box and fill into the tube for 3/4 full (about 75ml)
Use the stick to compress the ingredients inside the tube.
Cut the tin foil into small pieces and fold it twice to cover the open side of the tube.
Autoclave the filled tube at 120°C for 20 min.
Done!