May 22, 2024
Modelling Insulin resistance in vitro
- 1The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.
Protocol Citation: Laura Mahoney-Sanchez 2024. Modelling Insulin resistance in vitro. protocols.io https://dx.doi.org/10.17504/protocols.io.kxygxy3mwl8j/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 22, 2024
Last Modified: May 22, 2024
Protocol Integer ID: 100256
Keywords: ASAPCRN
Abstract
This protocol contains the instruction for modelling insulin resistance in iPSC-derived neurons by depriving the cells of insulin overnight followed by a 30 minutes insulin treatment the following day.
The media is removed from the cells
Cells are washed 1x with PBS
Fresh media is added the cells supplemented with B27 without insulin (Thermofisher A1895601).
Cells are left back in the incubator Overnight at 37 °C and 5% CO2
18h
The following day, insulin was added to the media at either 100 nanomolar (nM) , 250 nanomolar (nM) or 1 micromolar (µM) for 00:30:00
30m