Sep 23, 2023

Public workspaceMitophagy experiments

DOI
dx.doi.org/10.17504/protocols.io.n2bvj3yjnlk5/v1
  • 1Sascha Martens lab, University of Vienna, Max Perutz Labs - Vienna
Elias Adriaenssens
Open access
DOI: dx.doi.org/10.17504/protocols.io.n2bvj3yjnlk5/v1
Protocol Citation: Elias Adriaenssens 2023. Mitophagy experiments. protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvj3yjnlk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 04, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 84439
Keywords: Mitophagy experiments, ASAPCRN
Funders Acknowledgement:
Aligning Science Across Parkinson’s (ASAP)
Grant ID: ASAP-000350
Marie Skłodowska-Curie MSCA Postdoctoral fellowship
Grant ID: 101062916
Abstract
This protocol describes how to induce mitophagy in HeLa cells.
Attachments
772-1957.pdf
772-1957.pdf
32KB
Materials
Reagents

ReagentOligomycin AApexBio TechnologyCatalog #A5588

ReagentAntimycin A from Streptomyces sp.Merck MilliporeSigma (Sigma-Aldrich)Catalog #A8674

ReagentQ-VD-OPhApexBio TechnologyCatalog #A1901

Mitophagy experiments
Mitophagy experiments
8h
To induce mitophagy, treat cells with Concentration10 micromolar (µM) Oligomycin and Concentration4 micromolar (µM) Antimycin A.
In case cells are treated for more than Duration08:00:00 , add Concentration10 micromolar (µM) Q-VD-OPh to suppress apoptosis.
8h
Pipetting
Analyze the samples by SDS-PAGE and western blot or flow cytometry.
Analyze