Increase or decrease amount proportionally to prepare stock quantities as needed. Add dry reagents slowly to allow for dissolution in between each addition. Solutions will be prepared and autoclaved separately, except for carbon and vitamins which are filter sterilized through 0.2 micron PES membranes. Each autoclaved solution should be cooled completely before combining - remember to autoclave extra bottles or graduated cylinders as needed for measuring and mixing. The carbon source should only be added after autoclaved solutions have cooled; it is filter sterilized into the medium and can be added during mixing or at any time before setting up a culture. Extra solutions can be stored frozen or at 4 Celsius. This recipe is for preparing 1 L of liquid medium, but plates can be made as well with slight variation.