Oct 05, 2021
Microplate reader operating procedure
- 12021 iDEC NEFU_China
- NEFU_China 2021
Protocol Citation: Shuning Guo, Zhujun Wei 2021. Microplate reader operating procedure. protocols.io https://dx.doi.org/10.17504/protocols.io.bysspwee
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: October 05, 2021
Last Modified: October 05, 2021
Protocol Integer ID: 53810
Keywords: multimode microplate reader
Abstract
Use Tecan Spark® multimode microplate reader to measure absorbance or fluorescence intensity of green fluorescence protein.
Equipment
SPARK
NAME
Microwell plate reader
TYPE
TECAN
BRAND
SPARK
SKU
LINK
Materials
Tecan Spark® multimode microplate reader
Equipment
SPARK
NAME
Microwell plate reader
TYPE
TECAN
BRAND
SPARK
SKU
LINK
96-well plate
Safety warnings
Please read the instructions carefully before use.
Please turn off the power after use.
Before start
1. Click on "instrument" on the left, click on "temperature", and check "temperature control" to control the real-time temperature.
2. In the "Action" bar of the left column, you can drag it to the process list under the 96-well plate, drag up
and down to sort, click the trash can to delete the option. You can design your own processes that meet your needs.
Turn on the power and warm up for 10 minutes.
Open the program and select "New" to create a new process.
Select "Absorbance" in the left column. There is a schematic of a 96-well plate in the middle of the screen.
You can select the hole to be used according to the spotting hole, and the selected hole will turn blue.
Put in the 96-well plate, set the parameters, click "start" to measure the absorbance, and then remove the 96-well plate.
Wait a moment, it will generate an Excel file with results and various parameters. Save and copy the file to the u disk and process the data.