Observed colouration elements include six types of “spots” (Fig. 2; Table 1), i.e. round or irregular areas larger than one scale, darker than background, and with distinct margins; one preopercular “blotch”, i.e. a round area larger than a spot, darker than background, and with diffused and indistinct margins, typically overlapped with darker spots; and parr “marks”, i.e. vertical areas larger than spots and blotches, slightly darker than background, and with diffused and indistinct margins, typically overlapped with darker spots. In order to summarise individual colouration patterns, sets of elements are represented by lowercase italicised letters separated by “/”. Each pattern includes (i) three elements (m, f, d) on the scaleless area including the visible portion of the preopeculum, operculum, and cleithrum; (ii) five elements on the body in lateral view, except dorsal and ventral areas (m, f, d, r, p); (iii) ocellated spots (c); (iv) a preopercular blotch (b); and (v) parr marks (k) (Fig. 2; Table 1). In each set, the absence of an element is coded as 0. The ‘reference phenotype’ of a sexually mature individual of S. marmoratus is defined by the exclusive presence of marbled spots, i.e., as m00/m0000/0/0/0 (Fig. S5; Table S1). The S. marmoratus x S. trutta hybrid phenotype of a sexually mature individual is defined as either m**/m****/0/0/0, or ***/m****/0/0/0, or m**/*****/0/0/0, (where *= at least one of any element different than m). In order to minimise researcher effects, three different researchers examined the fish sample and coded the colouration patterns, and fish were re-examined to eliminate reading mismatches. Individual phenotypic and genotypic scores (values 0‒1) were obtained from coded colouration patterns and genetic data (Table S3; Note S1). A t-test was done, for testing whether the sample Pearson correlation between the phenotypic and genotypic scores differed significantly from 0.