Dehydration: briefly equilibrate spreads in 2X SSC (~3 sec) and immediately transfer to ascending alcohol gradients, 2 mins w/ lid on: 70% --> 85% --> 100% ethanol --> air-dry. While air-dry, prepare 10n µL of 1:5 probe master mix in hybridization buffer, e.g. In a colored PCR tube, add 2 µl of pre-warmed probes (Empire Genomics, stored in -20℃ protected from light), including all probes types, to 8 µl hybridization buffer (e.g. 1 µl each for 2 types of probes) and mix well by 3 pulses of vortex followed by a brief spin. For just DAPI staining, start from 28.