Jun 15, 2024

Public workspaceMetabolic programs drive function of therapeutic NK cells in hypoxic tumor environments

DOI
dx.doi.org/10.17504/protocols.io.5qpvo3y9xv4o/v1
  • 1University of Minnesota
Philippa R Kennedy
Open access
DOI: dx.doi.org/10.17504/protocols.io.5qpvo3y9xv4o/v1
Collection Citation: Philippa R Kennedy 2024. Metabolic programs drive function of therapeutic NK cells in hypoxic tumor environments. protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvo3y9xv4o/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: July 11, 2023
Last Modified: June 15, 2024
Collection Integer ID: 84864
Funders Acknowledgement:
Department of Defense
Grant ID: CA200922
Emily Whitehead Foundation
Grant ID: Nicole Gularte Fight for Cures
Abstract
A collection of protocols associated with the publication 'Metabolic programs drive function of therapeutic NK cells in hypoxic tumor environments' by Kennedy et al.
Files
Protocol
Degranulation and cytokine production (functional assay)
Name
Degranulation and cytokine production (functional assay)
Version 1
Philippa R Kennedy
Philippa R KennedyUniversity of Minnesota
Protocol
Isolation of natural killer (NK) cells from human blood products
Name
Isolation of natural killer (NK) cells from human blood products
Version 2
Philippa R Kennedy
Philippa R KennedyUniversity of Minnesota
Protocol
Assessment of oxidative phosphorylation and glycolysis in NK cells (Seahorse assays)
Name
Assessment of oxidative phosphorylation and glycolysis in NK cells (Seahorse assays)
Version 1
Philippa R Kennedy
Philippa R KennedyUniversity of Minnesota
Protocol
Expansion of NK cells on feeder cells
Name
Expansion of NK cells on feeder cells
Version 1
Philippa R Kennedy
Philippa R KennedyUniversity of Minnesota
Protocol
Quantification of synapse polarization ("the conjugate assay")
Name
Quantification of synapse polarization ("the conjugate assay")
Version 1
Philippa R Kennedy
Philippa R KennedyUniversity of Minnesota
Protocol
Proliferation assay
Name
Proliferation assay
Version 1
Philippa R Kennedy
Philippa R KennedyUniversity of Minnesota
Protocol
Mitochondrial staining of NK cells by flow cytometry
Name
Mitochondrial staining of NK cells by flow cytometry
Version 1
Philippa R Kennedy
Philippa R KennedyUniversity of Minnesota
Protocol
Time of flight mass cytometry (CyTOF)
Name
Time of flight mass cytometry (CyTOF)
Version 1
Philippa R Kennedy
Philippa R KennedyUniversity of Minnesota
Protocol
Adhesion assay
Name
Adhesion assay
Version 1
Philippa R Kennedy
Philippa R KennedyUniversity of Minnesota
Protocol
Cell line information
Name
Cell line information
Version 4
Philippa R Kennedy
Philippa R KennedyUniversity of Minnesota
Protocol
Time-lapse killing assay (monolayer - IncuCyte)
Name
Time-lapse killing assay (monolayer - IncuCyte)
Version 2
Philippa R Kennedy
Philippa R KennedyUniversity of Minnesota
Protocol
Confocal microscopy of intracellular components within NK cells
Name
Confocal microscopy of intracellular components within NK cells
Version 1
Philippa R Kennedy
Philippa R KennedyUniversity of Minnesota
Protocol
Impedance (xCELLigence)
Name
Impedance (xCELLigence)
Version 1
Philippa R Kennedy
Philippa R KennedyUniversity of Minnesota
Protocol
Assessing IL-15 bioavailability ("the bioassay")
Name
Assessing IL-15 bioavailability ("the bioassay")
Version 1
Philippa R Kennedy
Philippa R KennedyUniversity of Minnesota