A | B | C | D | |
Reagent | Volume | Supplier | Cat.number | |
DMEM without sodium pyruvate* | 500 ml | Invitrogen | 41965-039 | |
Fetal calf serum ** (13% final) | 75 ml | GlobePharm | ||
Non-essential amino acids, 100x | 5 ml | Invitrogen | 11140-035 | |
L-glutamine, 200mM, (100x) | 5 ml | Invitrogen | 25030-024 | |
b-mercaptoethanol, 50 mM | 0.6 ml | Invitrogen | 31350-010 | |
Penicillin/Streptomycin | 5 ml | Invitrogen | 15140-122 | |
LIF (ESGRO) (now we use home-made LIF-conditioned medium) | 1ml (check current titer and add accordingly) |
A | B | C | D | |
Reagent | Volume | Supplier | Cat.number | |
DMEM without sodium pyruvate* | 500 ml | Invitrogen | 41965-039 | |
Fetal calf serum** | 50 ml | GlobePharm | ||
Non-essential amino acids, 100x (optional) | 5 ml | Invitrogen | 11140-035 | |
L-glutamine, 200mM, (100x) | 5 ml | Invitrogen | 25030-024 | |
b-mercaptoethanol, 50 mM | 0.5 ml | Invitrogen | 31350-010 | |
Penicillin/Streptomycin | 5 ml | Invitrogen | 15140-122 |