Purpose: To better replicate the environment when performing mechanical dissociation, the COVARIS cyroPREP is used alongside the any Nuclei Isolation Protocol. Ultimately, the COVARIS cyroPREP machine pulverizes frozen tissue samples in order to prep for isolation of nuclei for single nucleus RNAseq. Using cyroPREP eliminates the need to mechanically dissociate tissue samples by hand with fine scissors, as well as variability of technicians.