Necessary materials are listed below, please see attached spreadsheet for more details.
DBC Quantification Materials List.xlsx Instrumentation/Large Equipment:
muffle oven: capable of heating glassware to 500ºC for 5 hours
drying oven: oven that can maintain 60ºC (optional but beneficial for evaporating methanol)
torch for flame sealing: capable of melting borosilicate glass
oxidation oven: programmable oven that can be set to 160ºC and hold steady for 6 hours
HPLC-DAD: must be able to run a 2-solution buffer gradient and detect absorbance at 240nm
6N Hydrochloric acid (Fischer SA56-4 ), Methanol (Fischer A4544), Acetone (Fischer A9294), 15.8N nitric acid (Fischer A200-212), 85% o-phosphoric acid (Fischer A260-500), sodium phosphate monohydrate (Fischer S369-500), sodium phosphate heptahydrate (Fischer S373-500), hemimellitic acid (TCI America H15925G), 1,3,5-benzenetricarboxylic acid (Thermo Scientific 105350500), 1,2,4-benzenetricarboxylic acid (Thermo Scientific 349030050), 1,2,4,5-benzenetetracarboxylic acid (Thermo Scientific 105311000), benzenepentacarboxylic acid (TCI America B095225G), benzenehexacarboxylic acid (TCI America B024625G)
High purity nitrogen (Airgas NI HP300) or Argon (Airgas AR UHP300) and/or zero air (Airgas AI Z300)*
*zero air cannot be exclusively used, you must have either high purity nitrogen and/or argon for this protocol
HPLC columns & SPE cartridges:
phenyl-hexyl column 4.6 x 150 mm, 2.7 µm (InfinityLab Poroshell 120 693975-912), phenyl-hexyl guard column 4.6 x 5 mm, 2.7 µm (InfinityLab Poroshell 120 820750-914), Bond Elut PPL cartridge 1g, 6mL (Aligent 12255002)
Drying Apparatus Materials:
Connectors/Adapters: one-way stopcock with male slip luer, adapter cap for 1, 3 and 6mL Bond Elut cartridges, five port manifold with female luer locks, male luer to hosebarb adapter (3/16"), male luer to male luer connector, female luer to hose barb adapter (3/32"), male luer lock to female luer,
Tubing: 3/16in Pump Tubing (Masterflex L/S 25), Pump Tubing (Masterflex L/S 16), nylon tubing 4mm OD, 2.5mm ID (Shimadzu 016-43284-01)
Other: sand, porcelain evaporating dishes, 5-gang valve aquarium pump accessory, hypodermic needles
Nalgene 890 tubing (1/16X1/8X1/32), male luer lock to hosebarb adapter (1/16"), pre-scored ampules 2mL (Wheaton 176776), serum vial racks (23.8 x 11.1 x 8.9 cm), SPE tube caps, female luer caps, 12-mL glass vials, mechanical pipettes (20-200uL and 100-1000uL), glass Pasteur pipettes, 2-mL vial rack, long steel forceps, 9mm wide opening glass thread vials (2-mL), 9mm autosampler inserts (375 uL), 9 mm autosampler vial screw thread caps