Oct 17, 2023
Masson-Fontana Staining
- 1Vall d'Hebron Research Institute
Protocol Citation: Miquel Vila 2023. Masson-Fontana Staining. protocols.io https://dx.doi.org/10.17504/protocols.io.ewov1q3opgr2/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 17, 2023
Last Modified: November 20, 2023
Protocol Integer ID: 89421
Abstract
Masson-Fontana Staining for melanin detection
Heat paraffin tissue sections at 60ºC for 10min.
Incubate sections in xylene (5 min, 3 times) and in ethanol serial washes (100-95-70%-H2O, 5 min each).
Incubate the sections with ammoniac solution for 40 min at 56ºC. Rinse sections in distilled water.
Incubate the sections with sodium thiosulphate for 2 min. Rinse sections in distilled water.
Finally, counterstain with Kernechtrot for 7 min. Rinse sections in distilled water.