License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 07, 2024
Last Modified: June 07, 2024
Protocol Integer ID: 101443
Abstract
This simple protocol maps ONT raw reads to Reference Sequences by Minimap2 and Samtools.
Install requirement software
Install requirement software
git clone https://github.com/lh3/minimap2
cd minimap2 && make
conda create -n samtools
conda activate samtools
conda config --add channels bioconda
conda config --add channels conda-forge
conda install -c bioconda samtools
Align reads to reference sequences (two options as bellow)
Align reads to reference sequences (two options as bellow)